Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
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Filtered Search Results
Tris(hydroxymethyl)aminomethane, USP, EP, BP, bioCERTIFIED™, 50 kg, Spectrum Chemical
Small and Specialty Supplier Partner
Small and/or specialty supplier based on Federal laws and SBA requirements.
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Small and/or specialty supplier based on Federal laws and SBA requirements.
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Gibco™ PBS, pH 7.4
Gibco PBS, pH 7.4, is a balanced salt solution ideal for cell culture applications. It lacks calcium, magnesium, and phenol red, preventing cell adhesion and assay interference. Trusted for quality, Gibco PBS ensures reliable results in cell washing, transport, and reagent preparation.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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| Physical Form | Liquid |
|---|---|
| pH | 7.3 to 7.5 |
| Manufacturing Quality | cGMP-compliant under the ISO 13485 standard |
| Chemical Name or Material | Phosphate Buffered Saline (PBS) |
| Recommended Storage | 15°C to 30°C |
| Concentration | 1 X |
| For Use With (Application) | Mammalian Cell Culture |
| Osmolality | 280 - 315 mOsm/kg |
| Sterility | Sterile-filtered |
Gibco™ DPBS, powder, calcium, magnesium
Dulbecco's Phosphate-Buffered Saline (DPBS) is a balanced salt solution used for a variety of cell culture applications, such as washing cells before dissociation, transporting cells or tissue, diluting cells for counting, and preparing reagents.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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| Includes | No Sodium Pyruvate |
|---|---|
| Physical Form | Powder |
| Manufacturing Quality | cGMP-compliant under the ISO 13485 standard |
| Chemical Name or Material | DPBS |
| For Use With (Application) | Mammalian Cell Culture |
| Solution Type | Dulbecco's Phosphate Buffered Saline |
| Product Line | Gibco™ |
Tris, Cell Culture Reagent, ≥99.95%, MP Biomedicals™
Tris, Cell Culture Reagent, ≥99.95% is a biological buffer for various applications.
MOPSO Sodium Salt, MP Biomedicals™
CAS: 79803-73-9 Molecular Formula: C7H14NNaO5S Molecular Weight (g/mol): 247.24 InChI Key: WSFQLUVWDKCYSW-UHFFFAOYNA-M IUPAC Name: sodium 2-hydroxy-3-(morpholin-4-yl)propane-1-sulfonate SMILES: [Na+].OC(CN1CCOCC1)CS([O-])(=O)=O
| CAS | 79803-73-9 |
|---|---|
| Molecular Weight (g/mol) | 247.24 |
| SMILES | [Na+].OC(CN1CCOCC1)CS([O-])(=O)=O |
| IUPAC Name | sodium 2-hydroxy-3-(morpholin-4-yl)propane-1-sulfonate |
| InChI Key | WSFQLUVWDKCYSW-UHFFFAOYNA-M |
| Molecular Formula | C7H14NNaO5S |
Novus Biologicals™ PBS Tablets
Small and Specialty Supplier Partner
Small and/or specialty supplier based on Federal laws and SBA requirements.
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Small and/or specialty supplier based on Federal laws and SBA requirements.
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Phosphate buffered saline tablets pH 7.4
MilliporeSigma™ TBS Tablets, Calbiochem™,
Dissolving one tablet in 500mL of deionized H2O yields 150mM NaCl, 50mM Tris-HCl buffer
MilliporeSigma™ PBS Tablets, Calbiochem™,
Dissolving one tablet in 1 liter of deionized H2O yields 140mM NaCl, 10mM phosphate buffer, and 3mM KCl
MilliporeSigma™ Calbiochem™ OmniPur™ TBE Buffer, 10X
Suitable for a wide range of molecular biology applications. Each liter of 10X TBE buffer yields 10 liters of 1X TBE buffer.
| Physical Form | Liquid |
|---|
MilliporeSigma™ Calbiochem™ OmniPur™ 100X TE Buffer Solution Liquid Concentrate
For use in molecular biology applications
Thermo Scientific Chemicals TRIS, 1.0M buffer soln., pH 7.5, 0.2 micron filtered
CAS: 77-86-1 Molecular Formula: C4H11NO3 Molecular Weight (g/mol): 121.136 MDL Number: MFCD00004679 InChI Key: LENZDBCJOHFCAS-UHFFFAOYSA-N PubChem CID: 6503 ChEBI: CHEBI:9754 IUPAC Name: 2-amino-2-(hydroxymethyl)propane-1,3-diol SMILES: C(C(CO)(CO)N)O
| PubChem CID | 6503 |
|---|---|
| CAS | 77-86-1 |
| Molecular Weight (g/mol) | 121.136 |
| ChEBI | CHEBI:9754 |
| MDL Number | MFCD00004679 |
| SMILES | C(C(CO)(CO)N)O |
| IUPAC Name | 2-amino-2-(hydroxymethyl)propane-1,3-diol |
| InChI Key | LENZDBCJOHFCAS-UHFFFAOYSA-N |
| Molecular Formula | C4H11NO3 |
Thermo Scientific Chemicals Phosphate-buffered saline (PBS, 10X), with Triton∣r X-100
Ideal for maintaining a constant pH.
| Physical Form | Liquid |
|---|---|
| Chemical Name or Material | Phosphate Buffered Saline (PBS) |
| Recommended Storage | Ambient temperatures |
| Concentration | 10 X |
Thermo Scientific Chemicals BIS-TRIS propane, 0.2M buffer soln., pH 8.0
CAS: 64431-96-5 Molecular Formula: C11H26N2O6 Molecular Weight (g/mol): 282.337 MDL Number: MFCD00004689 InChI Key: HHKZCCWKTZRCCL-UHFFFAOYSA-N PubChem CID: 125132 ChEBI: CHEBI:40947 IUPAC Name: 2-[3-[[1,3-dihydroxy-2-(hydroxymethyl)propan-2-yl]amino]propylamino]-2-(hydroxymethyl)propane-1,3-diol SMILES: C(CNC(CO)(CO)CO)CNC(CO)(CO)CO
| PubChem CID | 125132 |
|---|---|
| CAS | 64431-96-5 |
| Molecular Weight (g/mol) | 282.337 |
| ChEBI | CHEBI:40947 |
| MDL Number | MFCD00004689 |
| SMILES | C(CNC(CO)(CO)CO)CNC(CO)(CO)CO |
| IUPAC Name | 2-[3-[[1,3-dihydroxy-2-(hydroxymethyl)propan-2-yl]amino]propylamino]-2-(hydroxymethyl)propane-1,3-diol |
| InChI Key | HHKZCCWKTZRCCL-UHFFFAOYSA-N |
| Molecular Formula | C11H26N2O6 |