Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
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Filtered Search Results
ACES, Biological Buffer, 98%, Spectrum™ Chemical
Small and Specialty Supplier Partner
Small and/or specialty supplier based on Federal laws and SBA requirements.
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Small and/or specialty supplier based on Federal laws and SBA requirements.
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Gibco™ DPBS, no calcium, no magnesium
Gibco™ Balanced Salt Solutions are sterile-filtered, packaged in bioprocess containers, and ready to order.
| Color | Clear, colorless solution |
|---|---|
| Physical Form | Liquid |
| pH | 7.0 to 7.3 |
| Manufacturing Quality | cGMP |
| Chemical Name or Material | DPBS |
| Osmolality | 270 to 300 mOsm/kg |
| Sterility | Sterile-filtered |
Thermo Scientific Chemicals ACES, 99+%, for biochemistry
CAS: 7365-82-4 Molecular Formula: C4H10N2O4S Molecular Weight (g/mol): 182.194 MDL Number: MFCD00008030 InChI Key: DBXNUXBLKRLWFA-UHFFFAOYSA-N Synonym: N-(2-Acetamido)-2-aminoethanesulfonic acid,N-(Carbamoylmethyl)taurine PubChem CID: 81832 ChEBI: CHEBI:39062 IUPAC Name: 2-[(2-amino-2-oxoethyl)amino]ethanesulfonic acid SMILES: C(CS(=O)(=O)O)NCC(=O)N
| PubChem CID | 81832 |
|---|---|
| CAS | 7365-82-4 |
| Molecular Weight (g/mol) | 182.194 |
| ChEBI | CHEBI:39062 |
| MDL Number | MFCD00008030 |
| SMILES | C(CS(=O)(=O)O)NCC(=O)N |
| Synonym | N-(2-Acetamido)-2-aminoethanesulfonic acid,N-(Carbamoylmethyl)taurine |
| IUPAC Name | 2-[(2-amino-2-oxoethyl)amino]ethanesulfonic acid |
| InChI Key | DBXNUXBLKRLWFA-UHFFFAOYSA-N |
| Molecular Formula | C4H10N2O4S |
Gibco™ PBS, pH 7.4
Gibco PBS, pH 7.4, is a balanced salt solution ideal for cell culture applications. It lacks calcium, magnesium, and phenol red, preventing cell adhesion and assay interference. Trusted for quality, Gibco PBS ensures reliable results in cell washing, transport, and reagent preparation.
Non-distribution item offered as a customer accommodation; additional freight charges may apply.
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| Physical Form | Liquid |
|---|---|
| pH | 7.3 to 7.5 |
| Manufacturing Quality | cGMP-compliant under the ISO 13485 standard |
| Chemical Name or Material | Phosphate Buffered Saline (PBS) |
| Recommended Storage | 15°C to 30°C |
| Concentration | 1 X |
| For Use With (Application) | Mammalian Cell Culture |
| Osmolality | 280 - 315 mOsm/kg |
| Sterility | Sterile-filtered |
Tris, Cell Culture Reagent, ≥99.95%, MP Biomedicals™
Tris, Cell Culture Reagent, ≥99.95% is a biological buffer for various applications.
MilliporeSigma™ Tris Hydrochloride, OmniPur™, Calbiochem™,
CAS: 1185-53-1 Molecular Formula: C4H12ClNO3 Molecular Weight (g/mol): 157.594 InChI Key: QKNYBSVHEMOAJP-UHFFFAOYSA-N Synonym: 1,3-Propanediol, 2-amino-2-(hydroxymethyl)-, hydrochloride; 1,3-Propanediol, 2-amino-2- (hydroxymethyl)-, hydrochloride (1:1); 2-Amino-2-hydroxymethyl-1,3-propanediol hydrochloride; Tris(hydroxymethyl)aminomethane, hydrochloride; Tris hydrochloride PubChem CID: 93573 IUPAC Name: 2-amino-2-(hydroxymethyl)propane-1,3-diol;hydrochloride SMILES: C(C(CO)(CO)N)O.Cl
| PubChem CID | 93573 |
|---|---|
| CAS | 1185-53-1 |
| Molecular Weight (g/mol) | 157.594 |
| SMILES | C(C(CO)(CO)N)O.Cl |
| Synonym | 1,3-Propanediol, 2-amino-2-(hydroxymethyl)-, hydrochloride; 1,3-Propanediol, 2-amino-2- (hydroxymethyl)-, hydrochloride (1:1); 2-Amino-2-hydroxymethyl-1,3-propanediol hydrochloride; Tris(hydroxymethyl)aminomethane, hydrochloride; Tris hydrochloride |
| IUPAC Name | 2-amino-2-(hydroxymethyl)propane-1,3-diol;hydrochloride |
| InChI Key | QKNYBSVHEMOAJP-UHFFFAOYSA-N |
| Molecular Formula | C4H12ClNO3 |
Thermo Scientific Chemicals Phosphate-buffered saline (PBS, 5X), with EDTA
Helps to maintain a constant pH
| Physical Form | Liquid |
|---|---|
| Chemical Name or Material | Phosphate Buffered Saline (PBS) |
| Recommended Storage | Ambient temperatures |
| Concentration | 5 X |
Thermo Scientific Chemicals Tris(hydroxymethyl)aminomethane, Electrophoresis Grade, 99.5%
CAS: 77-86-1 Molecular Formula: C4H11NO3 Molecular Weight (g/mol): 121.136 MDL Number: MFCD00004679 InChI Key: LENZDBCJOHFCAS-UHFFFAOYSA-N Synonym: TRIS PubChem CID: 6503 ChEBI: CHEBI:9754 IUPAC Name: 2-amino-2-(hydroxymethyl)propane-1,3-diol SMILES: C(C(CO)(CO)N)O
| PubChem CID | 6503 |
|---|---|
| CAS | 77-86-1 |
| Molecular Weight (g/mol) | 121.136 |
| ChEBI | CHEBI:9754 |
| MDL Number | MFCD00004679 |
| SMILES | C(C(CO)(CO)N)O |
| Synonym | TRIS |
| IUPAC Name | 2-amino-2-(hydroxymethyl)propane-1,3-diol |
| InChI Key | LENZDBCJOHFCAS-UHFFFAOYSA-N |
| Molecular Formula | C4H11NO3 |
MOPSO, 0.2M buffer soln., pH 7.0, Thermo Scientific™
CAS: 79803-73-9 Molecular Formula: C7H14NNaO5S Molecular Weight (g/mol): 247.241 MDL Number: MFCD00070007 InChI Key: WSFQLUVWDKCYSW-UHFFFAOYSA-M PubChem CID: 23675131 IUPAC Name: sodium;2-hydroxy-3-morpholin-4-ylpropane-1-sulfonate SMILES: C1COCCN1CC(CS(=O)(=O)[O-])O.[Na+]
| PubChem CID | 23675131 |
|---|---|
| CAS | 79803-73-9 |
| Molecular Weight (g/mol) | 247.241 |
| MDL Number | MFCD00070007 |
| SMILES | C1COCCN1CC(CS(=O)(=O)[O-])O.[Na+] |
| IUPAC Name | sodium;2-hydroxy-3-morpholin-4-ylpropane-1-sulfonate |
| InChI Key | WSFQLUVWDKCYSW-UHFFFAOYSA-M |
| Molecular Formula | C7H14NNaO5S |
Thermo Scientific Chemicals BIS-TRIS propane, 0.2M buffer soln., pH 6.5
CAS: 64431-96-5 Molecular Formula: C11H26N2O6 Molecular Weight (g/mol): 282.337 MDL Number: MFCD00004689 InChI Key: HHKZCCWKTZRCCL-UHFFFAOYSA-N PubChem CID: 125132 ChEBI: CHEBI:40947 IUPAC Name: 2-[3-[[1,3-dihydroxy-2-(hydroxymethyl)propan-2-yl]amino]propylamino]-2-(hydroxymethyl)propane-1,3-diol SMILES: C(CNC(CO)(CO)CO)CNC(CO)(CO)CO
| PubChem CID | 125132 |
|---|---|
| CAS | 64431-96-5 |
| Molecular Weight (g/mol) | 282.337 |
| ChEBI | CHEBI:40947 |
| MDL Number | MFCD00004689 |
| SMILES | C(CNC(CO)(CO)CO)CNC(CO)(CO)CO |
| IUPAC Name | 2-[3-[[1,3-dihydroxy-2-(hydroxymethyl)propan-2-yl]amino]propylamino]-2-(hydroxymethyl)propane-1,3-diol |
| InChI Key | HHKZCCWKTZRCCL-UHFFFAOYSA-N |
| Molecular Formula | C11H26N2O6 |
MilliporeSigma™ MES, ULTROL™ Grade, Calbiochem™,
CAS: 4432-31-9 Molecular Formula: C6H13NO4S Molecular Weight (g/mol): 195.233 InChI Key: SXGZJKUKBWWHRA-UHFFFAOYSA-N Synonym: 2-(N-Morpholino)ethanesulfonic Acid PubChem CID: 78165 ChEBI: CHEBI:39005 IUPAC Name: 2-morpholin-4-ylethanesulfonic acid SMILES: C1COCCN1CCS(=O)(=O)O
| PubChem CID | 78165 |
|---|---|
| CAS | 4432-31-9 |
| Molecular Weight (g/mol) | 195.233 |
| ChEBI | CHEBI:39005 |
| SMILES | C1COCCN1CCS(=O)(=O)O |
| Synonym | 2-(N-Morpholino)ethanesulfonic Acid |
| IUPAC Name | 2-morpholin-4-ylethanesulfonic acid |
| InChI Key | SXGZJKUKBWWHRA-UHFFFAOYSA-N |
| Molecular Formula | C6H13NO4S |
Thermo Scientific Chemicals TRIS, 1.0M buffer soln., pH 6.5
CAS: 77-86-1 Molecular Formula: C4H11NO3 Molecular Weight (g/mol): 121.136 MDL Number: MFCD00004679 InChI Key: LENZDBCJOHFCAS-UHFFFAOYSA-N PubChem CID: 6503 ChEBI: CHEBI:9754 IUPAC Name: 2-amino-2-(hydroxymethyl)propane-1,3-diol SMILES: C(C(CO)(CO)N)O
| PubChem CID | 6503 |
|---|---|
| CAS | 77-86-1 |
| Molecular Weight (g/mol) | 121.136 |
| ChEBI | CHEBI:9754 |
| MDL Number | MFCD00004679 |
| SMILES | C(C(CO)(CO)N)O |
| IUPAC Name | 2-amino-2-(hydroxymethyl)propane-1,3-diol |
| InChI Key | LENZDBCJOHFCAS-UHFFFAOYSA-N |
| Molecular Formula | C4H11NO3 |
MilliporeSigma™ HEPES, Free Acid, OmniPur™, Calbiochem™,
CAS: 7365-45-9 Molecular Formula: C8H18N2O4S Molecular Weight (g/mol): 238.30 MDL Number: MFCD00006158 InChI Key: JKMHFZQWWAIEOD-UHFFFAOYSA-N Synonym: N-2-Hydroxyethylpiperazine-N'-2-ethanesulfonic acid PubChem CID: 23831 ChEBI: CHEBI:42334 IUPAC Name: 2-[4-(2-hydroxyethyl)piperazin-1-yl]ethane-1-sulfonic acid SMILES: OCCN1CCN(CCS(O)(=O)=O)CC1
| PubChem CID | 23831 |
|---|---|
| CAS | 7365-45-9 |
| Molecular Weight (g/mol) | 238.30 |
| ChEBI | CHEBI:42334 |
| MDL Number | MFCD00006158 |
| SMILES | OCCN1CCN(CCS(O)(=O)=O)CC1 |
| Synonym | N-2-Hydroxyethylpiperazine-N'-2-ethanesulfonic acid |
| IUPAC Name | 2-[4-(2-hydroxyethyl)piperazin-1-yl]ethane-1-sulfonic acid |
| InChI Key | JKMHFZQWWAIEOD-UHFFFAOYSA-N |
| Molecular Formula | C8H18N2O4S |
Thermo Scientific Chemicals MES, 1.0M buffer soln., pH 7.0
CAS: 145224-94-8 Molecular Formula: C6H15NO5S Molecular Weight (g/mol): 213.248 MDL Number: MFCD00283848 InChI Key: MIIIXQJBDGSIKL-UHFFFAOYSA-N PubChem CID: 16218417 IUPAC Name: 2-morpholin-4-ylethanesulfonic acid;hydrate SMILES: C1COCCN1CCS(=O)(=O)O.O
| PubChem CID | 16218417 |
|---|---|
| CAS | 145224-94-8 |
| Molecular Weight (g/mol) | 213.248 |
| MDL Number | MFCD00283848 |
| SMILES | C1COCCN1CCS(=O)(=O)O.O |
| IUPAC Name | 2-morpholin-4-ylethanesulfonic acid;hydrate |
| InChI Key | MIIIXQJBDGSIKL-UHFFFAOYSA-N |
| Molecular Formula | C6H15NO5S |