Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
- (1)
- (1)
- (3)
- (1)
- (107)
- (1)
- (2)
- (1)
- (2)
- (2)
- (69)
- (2)
- (1)
- (1)
- (1)
- (1)
- (10)
- (3)
- (2)
- (10)
- (6)
- (2)
- (4)
- (2)
- (10)
- (1)
- (5)
- (5)
- (110)
- (107)
- (1)
- (1)
- (1)
- (1)
- (1)
- (11)
- (2)
- (7)
- (1)
- (1)
- (1)
- (2)
- (6)
- (8)
- (23)
- (1)
- (12)
- (20)
- (1)
- (4)
- (1)
- (12)
- (1)
- (3)
- (1)
- (53)
- (6)
- (5)
- (34)
- (129)
- (2)
- (1)
- (26)
- (2)
- (1)
- (6)
- (1)
- (1)
- (3)
- (19)
- (2)
- (1)
- (26)
- (1)
- (1)
- (6)
- (7)
- (3)
- (8)
- (1)
- (86)
- (148)
- (8)
- (6)
- (10)
- (1)
- (1)
- (4)
- (1)
- (1)
- (1)
- (1)
- (1)
- (2)
- (2)
- (17)
- (143)
- (6)
- (3)
- (1)
- (3)
- (3)
- (7)
- (5)
- (3)
- (8)
- (1)
- (595)
- (5)
- (4)
- (4)
- (114)
- (23)
- (12)
- (48)
- (5)
- (4)
- (2)
- (5)
- (125)
- (2)
- (13)
- (10)
- (3)
- (14)
- (49)
- (1)
- (2)
- (31)
- (13)
- (26)
- (2)
- (4)
- (9)
- (9)
- (23)
- (45)
- (5)
- (55)
- (5)
- (13)
- (1)
- (3)
- (2)
- (3)
- (1)
- (9)
- (1)
- (94)
- (1)
- (3)
- (4)
- (4)
- (3)
- (3)
- (3)
- (10)
- (3)
- (31)
- (4)
- (3)
- (9)
- (3)
- (45)
- (1)
- (3)
- (1)
- (4)
- (1)
- (1)
- (2)
- (1)
- (1)
- (3)
- (5)
- (1)
- (4)
- (3)
- (5)
- (1)
- (10)
- (10)
- (6)
- (4)
- (2)
- (5)
- (3)
- (2)
- (5)
- (10)
- (1)
- (10)
- (1)
- (2)
- (3)
- (2)
- (2)
- (2)
- (5)
- (7)
- (1)
- (2)
- (10)
- (4)
- (2)
- (1)
- (1)
- (4)
- (3)
- (3)
- (1)
- (2)
- (1)
- (3)
- (3)
- (2)
- (13)
- (4)
- (1)
- (4)
- (3)
- (2)
- (2)
- (2)
- (1)
- (4)
- (2)
- (4)
- (3)
- (10)
- (1)
- (1)
- (1)
- (1)
- (14)
- (1)
- (38)
- (2)
- (1)
- (2)
- (9)
- (2)
- (3)
- (8)
- (2)
- (1)
- (2)
- (3)
- (1)
- (1)
- (1)
- (32)
- (1)
- (5)
- (8)
- (2)
- (14)
- (5)
- (1)
- (3)
- (5)
- (1)
- (22)
- (1)
- (62)
- (11)
- (1)
- (43)
- (1)
- (11)
- (2)
- (1)
- (2)
- (1)
- (9)
- (1)
- (38)
- (1)
- (1)
- (7)
- (1)
- (1)
- (5)
- (1)
- (2)
- (1)
- (1)
- (21)
- (5)
- (3)
- (1)
- (17)
- (3)
- (1)
- (4)
- (4)
- (1)
- (6)
- (1)
- (4)
- (77)
- (93)
- (3)
- (6)
- (21)
- (53)
- (1)
- (72)
- (1)
- (27)
- (1)
- (1)
- (51)
- (15)
- (5)
- (2)
- (29)
- (1)
- (1)
- (10)
- (5)
- (5)
- (4)
- (2)
- (1)
- (7)
- (12)
- (2)
- (7)
Filtered Search Results
MP Biomedicals™ MES SDS Running Buffer for Bis-Tris Gels, Powder
Powder MES buffer salts to make PAGE buffer.
HEPES, Biological Buffer, 99%, Spectrum™ Chemical
Small and Specialty Supplier Partner
Small and/or specialty supplier based on Federal laws and SBA requirements.
Learn More
Small and/or specialty supplier based on Federal laws and SBA requirements.
Learn More
CAS: 7365-45-9 Molecular Formula: C8H18N2O4S Molecular Weight (g/mol): 238.30 MDL Number: MFCD00006158 InChI Key: JKMHFZQWWAIEOD-UHFFFAOYSA-N IUPAC Name: 2-[4-(2-hydroxyethyl)piperazin-1-yl]ethane-1-sulfonic acid SMILES: OCCN1CCN(CCS(O)(=O)=O)CC1
| CAS | 7365-45-9 |
|---|---|
| Molecular Weight (g/mol) | 238.30 |
| MDL Number | MFCD00006158 |
| SMILES | OCCN1CCN(CCS(O)(=O)=O)CC1 |
| IUPAC Name | 2-[4-(2-hydroxyethyl)piperazin-1-yl]ethane-1-sulfonic acid |
| InChI Key | JKMHFZQWWAIEOD-UHFFFAOYSA-N |
| Molecular Formula | C8H18N2O4S |
MES, Monohydrate, cGMP Manufactured, 99.5%, Spectrum™ Chemical
Small and Specialty Supplier Partner
Small and/or specialty supplier based on Federal laws and SBA requirements.
Learn More
Small and/or specialty supplier based on Federal laws and SBA requirements.
Learn More
CAS: 145224-94-8 Molecular Formula: C6H15NO5S Molecular Weight (g/mol): 213.25 InChI Key: MIIIXQJBDGSIKL-UHFFFAOYSA-N IUPAC Name: 2-(morpholin-4-yl)ethane-1-sulfonic acid hydrate SMILES: O.OS(=O)(=O)CCN1CCOCC1
| CAS | 145224-94-8 |
|---|---|
| Molecular Weight (g/mol) | 213.25 |
| SMILES | O.OS(=O)(=O)CCN1CCOCC1 |
| IUPAC Name | 2-(morpholin-4-yl)ethane-1-sulfonic acid hydrate |
| InChI Key | MIIIXQJBDGSIKL-UHFFFAOYSA-N |
| Molecular Formula | C6H15NO5S |
HEPES, ≥99% (Dry Basis), Ultrapure
CAS: 7365-45-9 Molecular Formula: C8H18N2O4S Molecular Weight (g/mol): 238.30 MDL Number: MFCD00006158 InChI Key: JKMHFZQWWAIEOD-UHFFFAOYSA-N Synonym: N-[2-Hydroxyethyl]piperazine-N'-[2-Ethanesulfonic acid] PubChem CID: 23831 ChEBI: CHEBI:42334 IUPAC Name: 2-[4-(2-hydroxyethyl)piperazin-1-yl]ethanesulfonic acid SMILES: OCCN1CCN(CCS(O)(=O)=O)CC1
| PubChem CID | 23831 |
|---|---|
| CAS | 7365-45-9 |
| Molecular Weight (g/mol) | 238.30 |
| ChEBI | CHEBI:42334 |
| MDL Number | MFCD00006158 |
| SMILES | OCCN1CCN(CCS(O)(=O)=O)CC1 |
| Synonym | N-[2-Hydroxyethyl]piperazine-N'-[2-Ethanesulfonic acid] |
| IUPAC Name | 2-[4-(2-hydroxyethyl)piperazin-1-yl]ethanesulfonic acid |
| InChI Key | JKMHFZQWWAIEOD-UHFFFAOYSA-N |
| Molecular Formula | C8H18N2O4S |
Gibco™ PBS (10X), pH 7.4
Greener Choice
Offers one or more environmental benefits itemized in the U.S. FTC “Green Guides.”
Learn More
Offers one or more environmental benefits itemized in the U.S. FTC “Green Guides.”
Learn More
Gibco™ Balanced Salt Solutions are sterile-filtered, packaged in bioprocess containers, and ready to order.
| Includes | None |
|---|---|
| Color | Clear, colorless solution |
| Physical Form | Liquid |
| pH | 7.3 to 7.5 |
| Manufacturing Quality | cGMP |
| Chemical Name or Material | Phosphate Buffered Saline (PBS) |
| Concentration | 10X |
| Osmolality | 2800 to 3100 mOsm/kg |
| Sterility | Sterile-filtered |
MOPSO, 99.5%, MP Biomedicals™
CAS: 79803-73-9 Molecular Formula: C7H14NNaO5S Molecular Weight (g/mol): 247.241 InChI Key: WSFQLUVWDKCYSW-UHFFFAOYSA-M Synonym: sodium 2-hydroxy-3-morpholinopropane-1-sulfonate,mopso sodium salt,3-morpholino-2-hydroxypropanesulfonic acid sodium salt,3-n-morpholinyl-2-hydroxypropanesulfonic acid sodium salt,sodium 2-hydroxy-3-morpholin-4-yl propane-1-sulfonate,mopso sodium salt titration,mopso, 0.2m buffer solution, ph 6.0,mopso, 0.2m buffer solution, ph 6.5,mopso, 0.2m buffer solution, ph 7.0,mopso, 0.2m buffer solution, ph 7.5 IUPAC Name: sodium;2-hydroxy-3-morpholin-4-ylpropane-1-sulfonate
| CAS | 79803-73-9 |
|---|---|
| Molecular Weight (g/mol) | 247.241 |
| Synonym | sodium 2-hydroxy-3-morpholinopropane-1-sulfonate,mopso sodium salt,3-morpholino-2-hydroxypropanesulfonic acid sodium salt,3-n-morpholinyl-2-hydroxypropanesulfonic acid sodium salt,sodium 2-hydroxy-3-morpholin-4-yl propane-1-sulfonate,mopso sodium salt titration,mopso, 0.2m buffer solution, ph 6.0,mopso, 0.2m buffer solution, ph 6.5,mopso, 0.2m buffer solution, ph 7.0,mopso, 0.2m buffer solution, ph 7.5 |
| IUPAC Name | sodium;2-hydroxy-3-morpholin-4-ylpropane-1-sulfonate |
| InChI Key | WSFQLUVWDKCYSW-UHFFFAOYSA-M |
| Molecular Formula | C7H14NNaO5S |
MES, >98%, MP Biomedicals™
CAS: 145224-94-8 Molecular Formula: C6H15NO5S Molecular Weight (g/mol): 213.25 MDL Number: MFCD00149409 InChI Key: MIIIXQJBDGSIKL-UHFFFAOYSA-N Synonym: 2-[N-Morpholino]ethanesulfonic acid PubChem CID: 16218417 IUPAC Name: 2-(morpholin-4-yl)ethane-1-sulfonic acid hydrate SMILES: O.OS(=O)(=O)CCN1CCOCC1
| PubChem CID | 16218417 |
|---|---|
| CAS | 145224-94-8 |
| Molecular Weight (g/mol) | 213.25 |
| MDL Number | MFCD00149409 |
| SMILES | O.OS(=O)(=O)CCN1CCOCC1 |
| Synonym | 2-[N-Morpholino]ethanesulfonic acid |
| IUPAC Name | 2-(morpholin-4-yl)ethane-1-sulfonic acid hydrate |
| InChI Key | MIIIXQJBDGSIKL-UHFFFAOYSA-N |
| Molecular Formula | C6H15NO5S |
Tris, PlusOne™, Cytiva
CAS: 77-86-1 Molecular Formula: C4H11NO3 Molecular Weight (g/mol): 121.136 InChI Key: LENZDBCJOHFCAS-UHFFFAOYSA-N Synonym: Tris(hydroxymethyl)aminomethane PubChem CID: 6503 ChEBI: CHEBI:9754 IUPAC Name: 2-amino-2-(hydroxymethyl)propane-1,3-diol SMILES: C(C(CO)(CO)N)O
| PubChem CID | 6503 |
|---|---|
| CAS | 77-86-1 |
| Molecular Weight (g/mol) | 121.136 |
| ChEBI | CHEBI:9754 |
| SMILES | C(C(CO)(CO)N)O |
| Synonym | Tris(hydroxymethyl)aminomethane |
| IUPAC Name | 2-amino-2-(hydroxymethyl)propane-1,3-diol |
| InChI Key | LENZDBCJOHFCAS-UHFFFAOYSA-N |
| Molecular Formula | C4H11NO3 |
Thermo Scientific Chemicals TAPS, 99+%, for biochemistry
CAS: 29915-38-6 Molecular Formula: C7H17NO6S Molecular Weight (g/mol): 243.27 MDL Number: MFCD00007538 InChI Key: YNLCVAQJIKOXER-UHFFFAOYSA-N Synonym: 3-[Tris(hydroxymethyl)methylamino]-1-propanesulfonic acid PubChem CID: 121591 IUPAC Name: 3-[[1,3-dihydroxy-2-(hydroxymethyl)propan-2-yl]amino]propane-1-sulfonic acid SMILES: OCC(CO)(CO)NCCCS(O)(=O)=O
| PubChem CID | 121591 |
|---|---|
| CAS | 29915-38-6 |
| Molecular Weight (g/mol) | 243.27 |
| MDL Number | MFCD00007538 |
| SMILES | OCC(CO)(CO)NCCCS(O)(=O)=O |
| Synonym | 3-[Tris(hydroxymethyl)methylamino]-1-propanesulfonic acid |
| IUPAC Name | 3-[[1,3-dihydroxy-2-(hydroxymethyl)propan-2-yl]amino]propane-1-sulfonic acid |
| InChI Key | YNLCVAQJIKOXER-UHFFFAOYSA-N |
| Molecular Formula | C7H17NO6S |
MilliporeSigma™ PBS Tablets, Calbiochem™,
Dissolving one tablet in 1 liter of deionized H2O yields 140mM NaCl, 10mM phosphate buffer, and 3mM KCl
Corning™ Mediatech™ Cell Culture Phosphate Buffered Saline (10X)
Small and Specialty Supplier Partner
Small and/or specialty supplier based on Federal laws and SBA requirements.
Learn More
Small and/or specialty supplier based on Federal laws and SBA requirements.
Learn More
With low endotoxin content
TrisHydroxymethyl)Aminomethane ACS AR, Macron Fine Chemicals™
CAS: 77-86-1 Molecular Formula: C4H11NO3 Molecular Weight (g/mol): 121.136 InChI Key: LENZDBCJOHFCAS-UHFFFAOYSA-N PubChem CID: 6503 ChEBI: CHEBI:9754 IUPAC Name: 2-amino-2-(hydroxymethyl)propane-1,3-diol SMILES: C(C(CO)(CO)N)O
| PubChem CID | 6503 |
|---|---|
| CAS | 77-86-1 |
| Molecular Weight (g/mol) | 121.136 |
| ChEBI | CHEBI:9754 |
| SMILES | C(C(CO)(CO)N)O |
| IUPAC Name | 2-amino-2-(hydroxymethyl)propane-1,3-diol |
| InChI Key | LENZDBCJOHFCAS-UHFFFAOYSA-N |
| Molecular Formula | C4H11NO3 |
MilliporeSigma™ BIS-Tris, ULTROL™ Grade, Calbiochem™,
CAS: 6976-37-0 Molecular Formula: C8H19NO5 Molecular Weight (g/mol): 209.242 InChI Key: OWMVSZAMULFTJU-UHFFFAOYSA-N Synonym: [bis(2-Hydroxyethyl)imino]-tris(hydroxymethyl)methane PubChem CID: 81462 ChEBI: CHEBI:41250 IUPAC Name: 2-[bis(2-hydroxyethyl)amino]-2-(hydroxymethyl)propane-1,3-diol SMILES: C(CO)N(CCO)C(CO)(CO)CO
| PubChem CID | 81462 |
|---|---|
| CAS | 6976-37-0 |
| Molecular Weight (g/mol) | 209.242 |
| ChEBI | CHEBI:41250 |
| SMILES | C(CO)N(CCO)C(CO)(CO)CO |
| Synonym | [bis(2-Hydroxyethyl)imino]-tris(hydroxymethyl)methane |
| IUPAC Name | 2-[bis(2-hydroxyethyl)amino]-2-(hydroxymethyl)propane-1,3-diol |
| InChI Key | OWMVSZAMULFTJU-UHFFFAOYSA-N |
| Molecular Formula | C8H19NO5 |
MilliporeSigma™ TAE Buffer, 10X, Molecular Biology Grade, Calbiochem™,
A 10X concentrate that can be diluted to a 1X solution containing 40mM Tris, 40mM acetate, and 1mM EDTA
| Physical Form | Liquid |
|---|---|
| Recommended Storage | Ambient temperatures |
| Concentration | 20X |